Some of the most significant highlights of the team’s progress are as follows:
· Completed the first round of nanopore folding — nanopores act as a channel for outputting a signal when a target is detected.
· Tested various strand displacement methods — strand displacement is key for the modularity of the design, allowing the team to adapt the biosensor for different targets by changing attachments.
· Explored the activity of the aptazyme — aptazymes are engineered molecules that combine the binding capabilities of aptamers with the catalytic functions of enzymes. In the biosensor, they help to detect a target and cause a cleavage event, allowing for visible output.
· Created large unilamellar vesicles (LUVs) encapsulated with fluorescent dyes — LUVs are used as carriers for the visible indicator dye in our biosensor. Encapsulating dyes within LUVs allows for the controlled release and detection of fluorescent signals, which is essential for the accurate measurement of biomarker concentrations.